Comparison of Various Cryoprotective Agents on Washed Chicken Spermatozoa
نویسندگان
چکیده
منابع مشابه
Cryoprotective effect of different glycerol concentrations on domestic cat spermatozoa.
Cryopreservation of spermatozoa is a pivotal tool in assisted reproduction, and studies aiming to establish optimal freezing/thawing protocols are essential to enhance sperm survival. The objectives of the present study were to (1) compare the cryoprotective efficiency of three different glycerol concentrations (3%, 5%, and 7%) on the basis of post-thaw sperm quality and (2) investigate whether...
متن کاملCryoprotective effects of some amides on rabbit spermatozoa.
Semen was diluted 1:9 with egg yolk-citrate medium containing 0.31--3.1 M (final concentration) formamide, butyramide, acetamide, propionamide, dimethylformamide, lactamide, malomide, ethylene glycol, trimethylene glycol, dimethylsulphoxide (DMSO) or glycerol. After 30 min incubation at 20 degrees C, sperm motility was superior in hypertonic solutions of acetamide, lactamide, dimethylsulphoxid...
متن کاملEffects of various cryoprotective agents on the survival of unfrozen and frozen mouse embryos.
The effects of DMSO, ethylene glycol, glycerol, erythritol, dimethylformamide and sucrose on the survival of unfrozen and frozen mouse morulae were examined. All the agents had a deleterious effect on survival of the unfrozen morulae at 20 degrees C. The harmful effect of erythritol was lower at 0 degrees C and sucrose had a protective effect at that temperature. Dimethylformamide and DMSO were...
متن کاملEvaluation of Cryoprotective Agents for Use in the Cryopreservation of Equine Spermatozoa
The first equine pregnancy using sperm cryopreserved in glycerol was reported in 1957,1 and glycerol is still considered the optimal cryoprotectant for equine sperm.2–4 However, this opinion is largely based on data from other species, and research that evaluates additional cryoprotective agents (CPA’s) specifically for equine sperm is lacking. Other compounds such as ethylene glycol, dimethyl ...
متن کاملEffects of Various Cryoprotective Agents and Extender Osmolality on Post-thawed Ram Semen
The influence of different extender osmolality levels and the presence of different cryoprotectants on the postthawed semen’s characteristics and post-thawed plasma membrane integrity of ram spermatozoa were studied. Ram semen was frozen with TRIS-egg yolk based extender according to two-step dilution procedures. The final concentrations of the cryoprotectants: 6% glycerol, 6% 1,2propanediol, 6...
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ژورنال
عنوان ژورنال: Poultry Science
سال: 1974
ISSN: 0032-5791
DOI: 10.3382/ps.0530284